Dual ontogeny of disease-associated microglia and disease inflammatory macrophages in aging and neurodegeneration
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https://www.ncbi.nlm.nih.gov/sra/SRP363872
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Mice brain at different age were digested and cell suspension were prepared. Cells were stained using anti-CD45, F4/80, CD11b and Ly6C and sorted using an cell sorter BD ARIA. Macrophages were then prepared in order to perform single cell RNA-Seq Overall design: Brain macrophage CD45+F4/80+CD11b+Ly6C- heterogeneity was determined by single cell RNA-Seq analysis
创建时间：
2022-08-09
