Global changes of gene expression inuced by MIR31HG knockdown

Human adipose-derived stem cells (hASCs) are a highly attractive source in bone tissue engineering.We found that MIR31HG significantly promote the osteogenic differentiation of hASCs. However, the underlying mechanisms still await investigations. Thus the alteration of gene expression induced by MIR31HG knockdown was investigated. We used microarrays to detail the global programme of gene expression when MIR31HG was knocked down in hASCs and identified distinct classes of dn-regulated genes during this process. hASCs were transfected with shMIR31HG or the control vectors (shNC). Total RNA was isolated with Trizol from hASCs.