Gene expression profiling of neural crest cells from E10.5 mouse embryos with basal p53 or activated p53

To investigate the mechanisms by which p53 activation triggers developmental defects, we generated a mouse model in which p53 is activated in neural crest cells due to the expression of a mutant p53 protein (p53-25,26) that can bind to and stabilize wild-type p53. Here, we performed gene expression profiling on neural crest cells isolated from these embryos, to identify the transcriptional response to p53 activation during embryogenesis. RNA-seq was performed on neural crest cells isolated from E10.5 mouse embryos expressing a stabilized p53 protein (Wnt1Cre;Trp53LSL-25,26/+;R26mTmG/+) and littermate controls (Wnt1Cre;Trp53+/+;R26mTmG/+). n=5 embryos per genotype.为探究p53激活引发发育缺陷的分子机制，我们构建了一款小鼠模型：该模型中，因表达可结合并稳定野生型p53的突变型p53蛋白（p53-25,26），神经嵴细胞（neural crest cells）内的p53被激活。本研究针对从上述胚胎中分离得到的神经嵴细胞开展基因表达谱分析，以鉴定胚胎发育过程中p53激活所引发的转录应答。我们对表达稳定化p53蛋白的胚胎发育第10.5天（E10.5）小鼠胚胎（基因型为Wnt1Cre;Trp53LSL-25,26/+;R26mTmG/+）及其同窝对照（基因型为Wnt1Cre;Trp53+/+;R26mTmG/+）中分离得到的神经嵴细胞进行了RNA测序（RNA-seq）。每种基因型对应5枚胚胎。