Gene expression profiling of neural crest cells from E10.5 mouse embryos with basal p53 or activated p53

To investigate the mechanisms by which p53 activation triggers developmental defects, we generated a mouse model in which p53 is activated in neural crest cells due to the expression of a mutant p53 protein (p53-25,26) that can bind to and stabilize wild-type p53. Here, we performed gene expression profiling on neural crest cells isolated from these embryos, to identify the transcriptional response to p53 activation during embryogenesis. RNA-seq was performed on neural crest cells isolated from E10.5 mouse embryos expressing a stabilized p53 protein (Wnt1Cre;Trp53LSL-25,26/+;R26mTmG/+) and littermate controls (Wnt1Cre;Trp53+/+;R26mTmG/+). n=5 embryos per genotype.