Single cell RNA sequencing of lineage traced early postnatal myofibroblasts in the adult lung

Early postnatal myofibroblasts are a key cell type involved in postnatal alveolarization. The majority of these cells undergo apoptosis post-alveolarization. However, some dedifferntaite and persist in the adult lung. We utilized single cell RNA seq. to understand transcriptomics of these persisted myofibroblasts. Overall design: Acta2-CreERT2, ROSA26R(ZSgreen/+) mice were injected with 4-OH-TM on P6.5, P8.5 and P10.5, and at P60.5, single cell suspension of the lungs were made. The FACS sorted, ZSgreen+, cells were subjected to single cell RNA sequencing to assess marker expression in ZSgreen+ cells that were also SMA+ or SMA