Data from: Spatial and seasonal influences on culturable endophytic mycobiota associated with different tissues of Eugenia jambolana Lam. and their antibacterial activity against MDR strains

Background: Present study focuses on diversity and distribution analysis of endophytic fungi associated with different tissues of Eugenia jambolana. The influence of season and geographical location on diversity and distribution of endophytic fungi has been analyzed. Antibacterial activity of isolated fungal species has also been investigated against MDR bacterial strains. Result: A total of 1896 endophytic fungal isolates were obtained from healthy, surface sterilized tissues of leaf, stem and petiole tissues during summer, monsoon and winter season. Out of 24 fungal species isolated, 20 species belong to class Ascomycetes, 2 to Basidiomycetes and 2 to Zygomycetes. Maximum species diversity was in rainy season whereas colonization frequency was in winter. All the diversity indices showed maximum species diversity at site 5 (Yamunanager), rainy among the seasons and leaf among the tissues studied. Aspergillus genus was most frequently isolated. Aspergillus niger and Alternaria alternata were most dominant species. Three way ANOVA results showed that effect of season was highly significant on species diversity in relation to sites and tissues. 60% endophytic fungal extracts showed significant antibacterial activity against one or more than one MDR bacterial strain. Conclusion: Different fungal species were recovered from different sites but the inter-site comparisons were not significant according to Jaccard similarity coefficient. Diversity of such fungal endophytes indicates that Eugenia jambolana plant acts as an ecosystem facilitating survival of many microbes with impressive antibacterial potential.研究背景：本研究聚焦于乌墨（Eugenia jambolana）不同组织内生真菌的多样性与分布特征分析。本研究探讨了季节与地理位置对内生真菌多样性及分布的影响，并对分离得到的真菌菌株的抗耐多药（MDR）细菌活性进行了检测。 研究结果：本研究于夏季、雨季与冬季从健康且经表面灭菌的叶片、茎秆及叶柄组织中，共分离得到1896株内生真菌。在所分离得到的24个真菌物种中，20个隶属于子囊菌纲（Ascomycetes），2个隶属于担子菌纲（Basidiomycetes），2个隶属于接合菌纲（Zygomycetes）。物种多样性在雨季达到最高，而定殖率则在冬季最高。所有多样性指数均显示，5号样地（亚穆讷讷格尔，Yamunanager）的物种多样性最高，季节维度下以雨季最高，组织维度下以叶片最高。曲霉属（Aspergillus）为最常分离得到的类群，其中黑曲霉（Aspergillus niger）与细交链孢菌（Alternaria alternata）为优势物种。三因素方差分析（Analysis of Variance, ANOVA）结果表明，季节对样地与组织维度下的物种多样性均具有极显著影响。60%的内生真菌提取物对一种或多种耐多药（MDR）细菌菌株表现出显著的抗菌活性。 研究结论：不同样地均分离得到了不同的真菌物种，但基于雅卡尔相似性系数（Jaccard similarity coefficient）的样地间比较并未显示出显著差异。此类内生真菌的多样性表明，乌墨（Eugenia jambolana）可作为一种微生境，为多种具有优异抗菌潜力的微生物提供生存条件。