MsMYB62-like as a negative regulator of anthocyanin biosynthesis in <i>Malus spectabilis</i>

Crabapple is a valuable tree species in gardens due to its captivating array of flower and leaf colors, rendering it a favored choice in landscaping. The economic and ornamental values of <i>Malus</i> crabapple are closely associated with the biosynthesis of anthocyanin, a pigment responsible for its vibrant hues. The intricate regulation of anthocyanin biosynthesis involves the concerted activity of various genes. However, the specific mechanism governing this process in crabapple warrants in-depth exploration. In this study, we explored the inhibitory role of MsMYB62-like in anthocyanin biosynthesis. We identified <i>MsDFR</i> and <i>MsANS</i> as two downstream target genes of MsMYB62-like. These genes encode enzymes integral to the anthocyanin biosynthetic pathway. The findings demonstrate that MsMYB62-like directly binds to the promoters of <i>MsDFR</i> and <i>MsANS</i>, resulting in the downregulation of their expression levels. Additionally, our observations indicate that the plant hormone cytokinins exert a suppressive effect on the expression levels of <i>MsMYB62-like</i>, while concurrently upregulating <i>MsDFR</i> and <i>MsANS</i>. This study reveals that the MsMYB62-like-<i>MsDFR</i>/<i>MsANS</i> module plays an important role in governing anthocyanin levels in <i>Malus</i> crabapple. Notably, the regulatory interplay is modulated by the plant hormone cytokinins.

观赏海棠（Crabapple）因花、叶色彩绚丽多姿，是园林中极具价值的树种，广受景观造景领域的青睐。苹果属（<i>Malus</i>）观赏海棠的经济与观赏价值，与其花色叶色的呈色物质——花青素（anthocyanin）的生物合成紧密相关。花青素生物合成的复杂调控依赖于众多基因的协同作用，但目前针对观赏海棠中该调控过程的具体分子机制仍有待深入探究。本研究探究了MsMYB62-like在花青素生物合成中的抑制性功能，鉴定得到MsMYB62-like的两个下游靶基因<i>MsDFR</i>与<i>MsANS</i>，二者编码花青素生物合成通路中的关键功能酶。实验结果表明，MsMYB62-like可直接结合<i>MsDFR</i>与<i>MsANS</i>的启动子区域，进而下调二者的基因表达水平。此外，研究发现植物激素细胞分裂素（cytokinins）可抑制<i>MsMYB62-like</i>的表达，同时上调<i>MsDFR</i>与<i>MsANS</i>的转录水平。本研究揭示了MsMYB62-like-<i>MsDFR</i>/<i>MsANS</i>调控模块在苹果属观赏海棠花青素积累过程中的重要作用，且该调控通路可被植物激素细胞分裂素所介导调控。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于海棠花（Malus spectabilis）中花青素生物合成的调控机制，具体研究MsMYB62-like基因作为负调控因子的作用。研究发现MsMYB62-like通过直接结合下游靶基因MsDFR和MsANS的启动子，抑制其表达，从而降低花青素水平；同时，植物激素细胞分裂素能调控这一过程，抑制MsMYB62-like并上调靶基因。数据集包含相关实验的原始数据，支持对花青素合成分子机制的深入探索。