Molecular, culture, and immunohistologic methods for detection and isolation of B. quintana in blood meals, bed bugs, and their feces.

Group 1: 30 infected bed bugs adults; Group 2: 30 infected bed bugs instars L1; Group Control 1: 30 uninfected bed bugs adults; Control 2: 30 uninfected bed bugs instars L1No: number of bed bugs; (+) positive ; (-) negative ND: Not Done (because we haven’t enough feces to be cultured); (≈): approximatelyqPCR: Quantitative real-time polymerase chain reaction(‡) Infected or uninfected blood(*) observed in gut(¥) Confirmation by qPCRMolecular, culture, and immunohistologic methods for detection and isolation of B. quintana in blood meals, bed bugs, and their feces.