16S Sequencing of Human Gut Microbiome from faecal samples

Stool samples were collected from 76 pelvic cancer patients. The samples were self-collected by the patients using faecal collection paper and universal faecal collection containers. Bacterial community profiling of the human faecal samples was performed by sequencing of the 16S rRNA gene V1-V2 region on a MiSeq platform with v3 chemistry and 300bp paired-end reads. Region-specific primers including partial Illumina adapters were used for amplification of the V1-V2 region, followed by short cycle PCR addition of full-length Illumina adapters and dual barcodes. Resulting libraries were sequenced on the MiSeq platform.