Blue laser light induces nuclear accumulation of Dorsal-GFP expressed together with PND-HA-CactDN.
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The movie shows an embryo expressing Dorsal-GFP and PND-HA-CactDN imaged using the same conditions as described for S1 Movie from nc12 to nc14/gastrulation; importantly, including 20 min high power blue light (i.e. 488nm, 10%) illumination used to initiate degron-mediated loss of PND-HA-CactusDN activity at nc13/14, resulting in Dorsal-GFP nuclear localization. Snapshots from the movie are shown in Fig 9C–9C“‘. (ZIP)
创建时间：
2021-05-17
