Macrophage Lyn kinase is a Sex-Specific Regulator of Post-SAH Neuroinflammation

Lyn kinase is a member of the Src family of tyrosine kinases, primarily known for its role in regulating immune cell signaling. It can phosphorylate and modulate the activity of various proteins involved in immune responses, including TLR4. In our study, we investigated the sex-specific involvement of Lyn kinase in regulating TLR4 signaling to understand the TLR4 mediated inflammatory response after SAH. Our results highlight sex-specific modulation of Lyn kinase activity in TLR4 signaling after hemorrhagic stroke and indicate that successful treatment of neuroinflammation may require sex-specific treatments. Overall design: WT, MG-cKO and BMM-cKO three animal groups of both sexes (male and female) were used for spatial transcriptomics experiment. Each brain slices were assayed with Tmem119 (microglia) and Iba-1 (macrophage) markers using 4 ROIs each. Please note that while four groups (Sham, WT-SAH, MG-cKO, and BMM-cKO of both sexes with 4ROIs each) are included in the records (i.e. total 32 data samples), the analysis was performed between the 3 groups: WT-SAH, MG-cKO and BMM-cKO, which in total 24 data samples.