Rodent diversity and detection of Yersinia pestis in rodents. [Dataset 1. KaratuTz]

These rodent data were collected from Karatu district Tanzania for the purpose of assessing factors that may influence plague disease in plague foci villages and non-plague foci villages within the district. Rodents were captured with Sherman live animal traps and anaesthetized for collection of blood sample that were used to make blood smear on a glass slide for the purpose of observation of Yersinia pestis under compound light microscope and then were identified using a guide book for rodent identification. The euthanized rodent were then dissected and organs such as lungs and spleen were taken for confirmation of (pla) gene of Y.pestis DNA under qPCR technic following the results from the microscopic observation of stained blood smear

本数据集的啮齿类动物样本采集自坦桑尼亚卡拉图区，旨在评估该区域内鼠疫疫区与非疫区村落中，影响鼠疫发生的相关因素。研究人员采用薛曼活捕笼（Sherman live animal traps）捕获啮齿类动物，对其实施麻醉以采集血液样本；将血液滴涂于载玻片上制作血涂片，通过复合光学显微镜观察鼠疫耶尔森菌（Yersinia pestis），并依据啮齿类动物鉴定手册完成物种鉴定。待啮齿类动物安乐死后，研究人员对其进行解剖，采集肺、脾等器官样本，结合染色血涂片的显微镜镜检结果，通过实时荧光定量聚合酶链式反应（qPCR）技术，对鼠疫耶尔森菌的pla基因DNA进行验证。