Figure_3_Assimilatory_APS_PAPS_Reductase_Phylogeny

Figure 3 of manuscript "Comparative genomics and proteomic analysis of assimilatory sulfate reduction pathways in anaerobic methanotrophic archaea"<br>Files included:Fig3_APS_PAPS_reductase_clustalo_alignment.faa - ClustalO alignment of protein sequencesFig3_APS_PAPS_reductase_clustalo_alignment_filtered_for_tree.faa - Filtered protein sequences (172 amino acids) used for phylogeneticsFig3_APS_PAPS_Reductase_Phylogeny.png - Final Bayesian Phylogeny of Assimilatory APS/PAPS Reductases<br>Figure 3. Bayesian phylogeny of assimilatory adenyl-sulfate (APS) reductases and phosphoadenylyl-sulfate (PAPS) reductases. APS reductases and putative APS reductases are in green, PAPS reductases and putative PAPS reductases are in blue, bifunctional APS and PAPS reductase of Bacillus subtilis is in teal, and ANME proteins are bolded and in red. Archaeal and Bacterial/Eukaryal sequences formed separate clusters. Asterisks (*) indicate proteins that have been studied biochemically from Archaea (Cho, 2013; Lee et al., 2011), or Bacteria/Eukaryotes (Berndt et al., 2004; Gutierrez-Marcos et al., 1996; Kim et al., 2004; Savage et al., 1997; Suter et al., 2000; Yu et al., 2008). Length of the proteins ranged from 239 to 896 amino acids with the addition of protein domains. The protein domains, if found, are shown with filled symbols. Only 172 amino acid residues of the central shared region were used for phylogenetics. Given that two copies of APS/PAPS reductases were found in each ANME-2 lineage and clustered separately, it is likely one is for APS and the other is for PAPS reduction similar to M. jannaschii (Cho, 2013; Lee et al., 2011). Ca. Methanoperedens and four other methanogens in Methanosarcinales also contained a second putative assimilatory APS reductase more closely related to the bacterial/eukaryotic homologs, while ANME-1b contained a gene that does not cluster with assimilatory APS or PAPS reductases of known substrate. Protein accession numbers from the NCBI database or gene IDs from the IMG database are shown in parentheses. Black dots on the branches represent Bayesian posterior probability values greater than 90%, and scale bar indicates the number of amino acid substitutions per site.<br>