Fig3 a,b,c

(A) Flow cytometric analysis of ROS activity in Rad9+/+ and Rad9-/- MES cells exposed to 1G or SMG condition for 1 or 5days. (B) Flow cytometric analysis of ROS activity in Rad9-/-+Rad9 MES cells exposed to 1G or SMG condition for 1 or 5days. (C) N-acetylcysteine inhibited SMG-induced increase of ROS formation in Rad9-/- MES cells. Rad9-/- MES cells were mock-treated or treated with 0.05, 0.1 or 0.5 mM N-acetylcysteine under SMG for 1 day.(A) 流式细胞术分析暴露于1G重力或模拟微重力（Simulated Microgravity, SMG）环境1天或5天的Rad9野生型（Rad9+/+）与Rad9基因敲除（Rad9-/-）MES细胞的活性氧（Reactive Oxygen Species, ROS）活性。(B) 流式细胞术分析暴露于1G重力或模拟微重力环境1天或5天的回补Rad9的Rad9基因敲除（Rad9-/-+Rad9）MES细胞的活性氧活性。(C) N-乙酰半胱氨酸（N-acetylcysteine, NAC）可抑制模拟微重力诱导的Rad9基因敲除MES细胞中活性氧生成增加。Rad9基因敲除MES细胞在模拟微重力环境下培养1天，分别接受空白对照处理或0.05、0.1、0.5 mM的N-乙酰半胱氨酸处理。