<i>Amanita fulvogrisea </i>Illescassp. nov., alignement, ML and BI trees.
收藏资源简介:
<i>Amanita fulvogrisea</i> Illescas, <i>sp</i>. <i>nov</i>. Crous PW, Costa MM, Kandemir H, et al. 2023. Fungal Planet description 1551 – 1613. <br>DOI: https://doi.org/10.3767/persoonia.2023.51.08Maximum likelihood (ML) tree in Nexus format obtained from the combination of 40 ITS + LSU sequences, showing in the branches the ML bootstrap support (ML-bs) calculated with IQ-TREE v. 2.1.3 (Nguyen et al. 2015), performing 300 standard bootstrap replicates, and applying the models selected with ModelFinder for each of the partitions used: TPM2+F+G4 for ITS, and TN+F+I for LSU.Bayesian inference (BI) tree in Nexus format obtained from the combination of 40 ITS + LSU sequences, showing in the branches the Bayesian posterior probability (BPP) calculated with MrBayes v. 3.2 (Ronquist et al. 2012), performing 1000000 generations, and applying the models selected with ModelFinder (S. Kalyaanamoorthy, B.Q. Minh, T.K.F. Wong, A. von Haeseler, L.S. Jermiin<b> </b>(2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. <i>Nat. Methods</i>, 14:587-589) for each of the partitions used: TPM2+F+G4 for ITS, and TN+F+I for LSU.Nexus format alignment of 30 taxa of <i>Amanita</i> section <i>Vaginatae</i>, Clade 5 obtained with Mafft online, L-ins-i strategy (Katoh, Rozewicki, Yamada 2019 (Briefings in Bioinformatics 20:1160-1166). MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization), and manually adjusted with Bioedit 7.2.5 (Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser. 41:95-98).



