Data from: Reward-induced phasic dopamine release in the monkey ventral striatum and putamen

In-vivo voltammetry has successfully been used to detect dopamine release in rodent brains, but its application to monkeys has been limited. We have previously detected dopamine release in the caudate of behaving Japanese monkeys using diamond microelectrodes (Yoshimi 2011); however it is not known whether the release pattern is the same in various areas of the forebrain. Recent studies have suggested variations in the dopaminergic projections to forebrain areas. In the present study, we attempted simultaneous recording at two locations in the striatum, using fast-scan cyclic voltammetry (FSCV) on carbon fibers, which has been widely used in rodents. Responses to unpredicted food and liquid rewards were detected repeatedly. The response to the liquid reward after conditioned stimuli was enhanced after switching the prediction cue. These characteristics were generally similar between the ventral striatum and the putamen. Overall, the technical application of FSCV recording in multiple locations was successful in behaving primates, and further voltammetric recordings in multiple locations will expand our knowledge of dopamine reward responses.活体伏安法（in-vivo voltammetry）已成功应用于啮齿类动物脑内多巴胺释放的检测，但该技术在猴类中的应用仍较为受限。我们此前曾利用金刚石微电极，在行为态日本猴的尾状核中检测到多巴胺释放（Yoshimi, 2011）；然而目前尚不清楚前脑不同区域的多巴胺释放模式是否一致。近期已有研究表明，投射至前脑各区域的多巴胺能神经通路存在差异。本研究中，我们采用已广泛应用于啮齿类动物研究的、基于碳纤维电极的快速扫描循环伏安法（fast-scan cyclic voltammetry, FSCV），尝试对纹状体的两个位点进行同步记录。研究中反复检测到动物对未预期的食物与液体奖赏的响应；在切换预测线索后，条件刺激引发的液体奖赏响应得到增强。腹侧纹状体与壳核的上述响应特征整体相似。综上，FSCV多位点记录技术在行为态灵长类动物中的应用取得成功，未来开展更多多位点伏安法记录研究，将进一步深化我们对多巴胺奖赏响应机制的认知。