Profiling the secondary metabolite potential of rhizospheric Streptomyces sp. 7H through multi-Omics analysis

The increase in antimicrobial resistance (AMR) highlights the urgent need for the discovery of novel antimicrobial compounds. Streptomyces species are promising sources for bioactive compounds due to their intrinsic capacity for secondary metabolite production. This study investigates the secondary metabolite potential of Streptomyces sp. 7H, which was isolated from the rhizosphere of the wild medicinal plant Malva neglecta in Hamadan Province, Iran. Utilizing multi-omics approaches including genomics, proteomics, and metabolomics, we explored the genetic and biochemical characteristics of strain 7H. Genome sequencing revealed a linear chromosome of 8,359,082 bp in a potential novel Streptomyces species and identified 46 putative biosynthetic gene clusters (BGCs), indicating significant potential for diverse secondary metabolite production. The production of collismycin A (CMA) that exhibited antimicrobial activity against indicator strains Bacillus subtilis and Stenotrophomonas maltophilia, was confirmed through mass spectrometry analysis of metabolites, . Furthermore, proteomic analysis supported the expression of genes associated with antibiotic biosynthesis and resistance.