Brain–muscle tissue communication prevents muscle aging by maintaining daily physiology

The muscle peripheral clock selects, suppresses, and integrates signals from the central clock that serve muscle homeostasis, thus revealing its critical gatekeeper function. Overall design: Four biological replicates (2 males and 2 females, if not otherwise specified) at each zeitgeber time (ZT) point were used to collect RNA samples from the snap-frozen tibialis anterior muscle. A total of 360 mouse RNA samples were sequenced to generate the circadian RNA transcriptome. Eight samples were excluded from analysis due to quality issues.