RNA-Seq of Anopheles coluzzii midguts infected with Plasmodium berghei before and during midgut invasion.. Anopheles coluzzii and Plasmodium berghei (c507wt, Δc01 and Δc57) transcriptional responses during midgut invasion.

The availability of the genome sequence of the African malaria vector An. coluzzii (Anopheles gambiae M molecular form) has opened new opportunities to dissect at the molecular level the vector-pathogen interactions leading to malaria transmission. Here, we describe the characterization of the genome-wide transcriptional responses of An. coluzzii to infection with wild-type, Δc01 and Δc57 Plasmodium berghei laboratory isolates. Colonized An. coluzzii mosquitoes were fed on anesthetized mice infected with P. berghei, and the abundance of transcripts in the mosquito midgut was monitored at 1hpi and 24hpi.