DNA methylation in developing fetal brain of pig

DNA methylation was profiled in a genome-wide manner by Enzymatic Methyl-seq (EM-seq) in fetal brain of both sexes at three gestation days 45, 60 and 90. The analysis showed nearly 3-fold fewer differentially methylated sites (DMSs) between GD90 and GD60 (n=54) compared to that between GD60 and GD45 (n=150). Comparative analysis of the methylation data with the RNA-seq data previously generated from the same gestation times showed an opposition pattern where more number of differentially expressed genes (DEGs) were observed between GD90 and GD60 compared to GD60 and GD45. Overall design: DNA methylation profiling of all 12 samples (6 fetal brain samples and 6 adult blood samples) by enzymatic methyl-seq (EM-seq).