High-Throughput Proteomics Processing of Proteins in Polyacrylamide in a Multiwell Format

Processing multiple protein samples from polyacrylamide at significant sensitivity represents a major
chokepoint for raising the success rate in high-volume
protein identification projects. A multiwell filterplate
method for processing proteins in polyacrylamide was
optimized for sensitivity using a protein standard. The
results demonstrate this process to be a reliable and
reproducible method over a range of gel loadings and
suitable for the identification of proteins near the threshold of silver stain. This high-throughput manual method
requires a minimum of specialized equipment, and can
be performed disconnected from a proteomics infrastructure for the preparation of mass spectrometry-ready
samples.
Keywords: Polyacrylamide • Proteomics • Multiwell • Trypsin •
Extraction