Regulation of acupuncture on genes in brain stem

Recent clinical trials have shown that hand acupuncture improves cognition and other clinical outcomes of central nerve injuries including TBI,and we would like to observe that the effects of acupuncture on key signaling pathways and neuronal networks of the brainstem in TBI rats to promote the recovery of consciousness. We used microarray to detail the expression of wake-up related genes in brainstem The 1h brainstem samples (n=5) were mixed, extracted, purified, whose qualities were tested for total RNA with a standard Affymetrix protocol according to Shanghai Biotechnology Corporation (Shanghai, China) as reported previously.