Distribution of mutations in evolved syntrophic co-cultures of sulfate reducing bacteria Desulfovibrio vulgaris Hildenborough and methanogenic archaea Methanococcus maripaludis S2

Synthetically established syntrophic cultures of Desulfovibrio vulgaris Hildenborough and Methanococcus maripaludis S2 were cultivated in two different evolution environments (shaking vs no-shaking) for 1000 generations. DNA samples were collected and subjected to whole genome sequencing to be able to identify variations accumulated during the evolution of syntrophy in both species.