Characterization of organ-specific regulatory B cells using single-cell RNA sequencing. Characterization of organ-specific regulatory B cells using single-cell RNA sequencing

We characterized Breg cells by scRNA-seq based on the immunosuppressive gene expression profile and B10 related genes, and delineated their transcriptional profiles, underlying functions and BCR clonotypes in different mouse organs under physiological condition. Overall design: B cells sorted from liver, spleen, mLN, BM and PC of four wild type mice