Presence of CD3+ and CD79a+ lymphocytes in the pituitary gland of dogs at post mortem examination

Dataset accompanying the paper "Presence of CD3+ and CD79a+ lymphocytes in the pituitary gland of dogs at post mortem examination".

The dataset consists of a comma-separated values (CSV) file with data from 20 dogs recruited at routine necropsy to investigate presence and distribution of lymphocytes in pituitary glands in dogs with no suspicion of pituitary disease. The pituitary glands were collected at the Section of Pathology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, from November 2013 to February 2014, and April 2017 to September 2017. Formalin-fixed paraffin-embedded pituitary sections were stained with haematoxylin and eosin (H&E) or subjected to immunohistochemistry (IHC) using primary antibodies specific for the T-cell marker CD3 and B-cell marker CD79a. The number of CD3+ and CD79a+ cells per area unit (CPA, cells/mm²) was determined for different pituitary regions: pars tuberalis (PT), pars distalis (PD), pars intermedia (PI), infundibulum (In), and lobus nervosus (LN).

The dataset contains information about:

• Identification and signalement — fields: ID, Breed, Age (years of age at necropsy), Sex, Neutering status

• Concurrent pathological findings — field: Necropsy diagnosis (multiple diagnoses are separated by semicolon)

• Morphologic changes in the pituitary gland — fields: Pituitary lesion, Pituitary lesion group (No, Limited or Extensive lesion)

• Number of CD3+ CPA (cells/mm²) in each pituitary region* — fields: PT CD3 CPA, PD CD3 CPA, PI CD3 CPA, In CD3 CPA, LN CD3 CPA

• Number of CD79a+ CPA (cells/mm²) in each pituitary region* — fields: PT CD79a CPA, PD CD79a CPA, PI CD79a CPA, In CD79a CPA, LN CD79a CPA

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*PT, pars tuberalis; PD, pars distalis; PI, pars intermedia; In, infundibulum; LN, lobus nervosus

本数据集配套发表于论文《犬垂体腺尸检样本中CD3+与CD79a+淋巴细胞的存在情况》。
本数据集包含一份逗号分隔值（comma-separated values, CSV）格式文件，其数据源自20只常规尸检的犬只，用于探究无垂体疾病疑似史的犬垂体腺内淋巴细胞的存在状态与分布特征。
垂体腺样本采集于2013年11月至2014年2月、2017年4月至2017年9月期间，采集地点为瑞典农业科学大学（Swedish University of Agricultural Sciences）乌普萨拉分校生物医学科学与兽医公共卫生系病理教研室。
将采集到的垂体腺组织经福尔马林固定、石蜡包埋后制作切片，分别采用苏木精-伊红（haematoxylin and eosin, H&E）染色，或使用针对T细胞标志物CD3与B细胞标志物CD79a的一抗开展免疫组织化学（immunohistochemistry, IHC）染色。针对垂体腺的5个区域：结节部（pars tuberalis, PT）、远侧部（pars distalis, PD）、中间部（pars intermedia, PI）、漏斗部（infundibulum, In）以及神经叶（lobus nervosus, LN），测定每单位面积内CD3+与CD79a+细胞的数量（CPA，cells/mm²）。

本数据集包含以下几类信息：
• 个体识别与基础信息：字段包括ID、品种、年龄（尸检时的实际年龄，单位：岁）、性别、绝育状态
• 伴随病理学发现：字段为尸检诊断（多项诊断以分号分隔）
• 垂体腺形态学改变：字段包括垂体病变情况、垂体病变分级（分为无病变、局限性病变或广泛性病变）
• 各垂体区域内CD3+细胞的单位面积计数（cells/mm²）：字段依次为PT CD3 CPA、PD CD3 CPA、PI CD3 CPA、In CD3 CPA、LN CD3 CPA
• 各垂体区域内CD79a+细胞的单位面积计数（cells/mm²）：字段依次为PT CD79a CPA、PD CD79a CPA、PI CD79a CPA、In CD79a CPA、LN CD79a CPA

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*注：PT即结节部（pars tuberalis）；PD即远侧部（pars distalis）；PI即中间部（pars intermedia）；In即漏斗部（infundibulum）；LN即神经叶（lobus nervosus）