Electrochemical immunosensors using electrodeposited gold nanostructures for detecting the S proteins from SARS-CoV and SARS-CoV-2

This data is related to the development of an electrochemical immunosensor for the S protein of SARS-CoV and SARS-CoV-2 viruses. For that, screen-printed carbon electrodes (SPCEs) were modified by the electrodeposition of gold nanoparticles using different conditions. Specific anti-S protein antibodies were immobilized in the surface of the electrode using EDC and NHS, with the presence of the analyte being assessed by electrochemical impedance spectroscopy. Immunsensors were tested using biological samples, showing great potential to be applied in clinical samples. Furthermore, the devices were able to detect S protein in a rapid (35 min), sensitive and low-cost (< US$ 0.03 per device) manner.

本数据集关联针对严重急性呼吸综合征冠状病毒（SARS-CoV）与新型冠状病毒（SARS-CoV-2）刺突（S）蛋白开发的电化学免疫传感器研究。研究中，研究人员采用不同工艺条件，通过电沉积法对丝网印刷碳电极（screen-printed carbon electrodes, SPCEs）实施金纳米粒子修饰；随后借助1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐（EDC）与N-羟基琥珀酰亚胺（NHS），将特异性抗S蛋白抗体固定于电极表面，并通过电化学阻抗光谱法检测分析物的存在状态。该免疫传感器经生物样本测试验证，结果表明其在临床样本检测场景中具备极佳应用潜力。此外，该检测器件可实现快速（35分钟）、高灵敏且低成本（单器件成本低于0.03美元）的S蛋白检测。