Qualitative proteomics analysis of the chicken amniotic fluid

We designed a bottom-up proteomic approach combining two complementary strategies as GeLC-MS/MS (protein samples fractionated by SDS-PAGE are analyzed by nanoLC-MS/MS after in-gel digestion) and shotgun (protein samples are directly analyzed by nanoLC-MS/MS after in-solution digestion) to give an exhaustive view of the proteome of the chicken amniotic fluid at the 11th day of the development, before the substantial transfer of egg white (which completely changes the global protein profile).