Peptide profiling of amniotic fluid samples from CAKUT fetuses

Summary

Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) is the leading cause of childhood end-stage renal disease and a significant cause of chronic kidney disease in adults. Genetic and environmental factors have been shown to influence CAKUT development, but the known disease mechanism remains incomplete. Our goal is to identify affected pathways and networks in CAKUT, through multi-omics analysis, including the peptidome. The peptidome data published here correspond to the raw data of the previously published dataset on postnatal kidney survival [pmid: 32750455].  The miRNome and proteome [pmid: 33987838] data are available in another repository and pride respectively.

Study design

Amniotic fluid samples were collected in a prospective multicenter observational study focusing on fetal bilateral CAKUT (part of a clinical trial). In addition 21 samples were collected from non-CAKUT individuals. The severity was defined based on the renal status after 2 years of postnatal follow-up. Amniotic fluid was used to extract peptides for LC-MS/MS and CE-MS/MS analysis. The complete protocol can be found in the supplementals.