Complete blood count reference intervals from a healthy adult urban population in Kenya

Background: There are racial, ethnic and geographical differences in complete blood count (CBC) reference intervals (RIs) and therefore it is necessary to establish RIs that are population specific. Several studies have been carried out in Africa to derive CBC RIs but many were not conducted with the rigor recommended for RI studies hence limiting the adoption and generalizability of the results. Method: By use of a Beckman Coulter ACT 5 DIFF CP analyser, we measured CBC parameters in samples collected from 528 healthy black African volunteers in a largely urban population. The latent abnormal values exclusion (LAVE) method was used for secondary exclusion of individuals who may have had sub-clinical diseases. The RIs were derived by both parametric and non-parametric methods with and without LAVE for comparative purposes. Results: Haemoglobin (Hb) levels were lower while platelet counts were higher in females across the 4 age stratifications. The lower limits for Hb and red blood cell ...背景：全血细胞计数（complete blood count, CBC）的参考区间（reference intervals, RIs）存在种族、族群及地理差异，因此有必要建立针对特定人群的参考区间。目前已有多项在非洲开展的研究旨在推导全血细胞计数参考区间，但其中多数未遵循参考区间研究推荐的严谨标准，因此限制了其研究结果的推广与普适性。 方法：本研究使用贝克曼库尔特（Beckman Coulter）ACT 5 DIFF CP血液分析仪，对采集自528名健康非洲黑人志愿者的样本进行全血细胞计数参数检测，该研究人群以城市人口为主。本研究采用潜在异常值排除（latent abnormal values exclusion, LAVE）法，对可能存在亚临床疾病的个体进行二次排除。为便于对比分析，本研究分别采用参数法与非参数法，在使用与未使用LAVE法的情况下推导参考区间。 结果：在4个年龄分层中，女性的血红蛋白（Haemoglobin, Hb）水平均低于男性，而血小板计数则更高。血红蛋白与红细胞的下限值……