Explored the underlying mechanism by which ILF3 exerted carcinogenesis and its potential targets in MM. Explored the underlying mechanism by which ILF3 exerted carcinogenesis and its potential targets in MM

An RIP experiment was performed in ARP1 cell following the instructions of the article. ~5-20 × 10^6 cells were harvested and lysed. Protein A/G MagBeads pre-coated with 5μg of the antibody of interest (HNRNPA2B1/ILF3, Proteintech) and incubated with cell lysate supernatant overnight at 4°C.The beads containing immunoprecipitated RNA-protein complex were treated with 150μL of Proteinase K buffer to digest the proteins. Specific binding RNAs were isolated by using TRIzol. Overall design: Examination in ARP1 cell.