Frequency of colony formation by regrowth cells after senescence.
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aTotal population doubling levels (TPDL) show the number of cell division that cells divided until reaching to senescence.bFrequency of colony formation by regrowth cells in three P100 dishes after senescence. Briefly, 1.5×106 cells were inoculated into three P100 dishes. Then, cells were incubated in a 100% humidified CO2 incubator at 37°C for 6 weeks with medium change every 3 days. Regrowth colonies were counted. Data show the mean ± S.D. of six independent experiments.
创建时间：
2015-12-02
