Data for: Link between glucose metabolism and EMT drives triple negative breast cancer migratory heterogeneity

Intracellular and environmental cues result in heterogeneous cancer cell populations with different metabolic and migratory behaviors. While glucose metabolism and EMT have previously been linked, we aim to understand how this relationship fuels cancer cell migration. We show that while glycolysis drives single-cell migration in confining microtracks, fast and slow cells display different migratory sensitivities to glycolysis and oxidative phosphorylation inhibition. Phenotypic sorting of highly and weakly migratory subpopulations (MDA+, MDA-) reveals that more mesenchymal, highly migratory MDA+ preferentially use glycolysis while more epithelial, weakly migratory MDA- utilize mitochondrial respiration. These phenotypes are plastic and MDA+ can be made less glycolytic, mesenchymal, and migratory and MDA- can be made more glycolytic, mesenchymal, and migratory via modulation of glucose metabolism or EMT. These findings reveal an intrinsic link between EMT and glucose metabolism that cont..., Data was collected using equipment and materials in the Reinhart-King lab. Studies were conducted as described in STAR Methods section of publication. Data were processed using Fiji/ImageJ and recorded in Excel., Microsoft excel is required.细胞内信号与环境刺激可诱导产生具有差异化代谢与迁移行为的异质性癌细胞群。尽管既往研究已证实糖代谢与上皮间质转化（Epithelial-Mesenchymal Transition, EMT）之间存在关联，本研究旨在阐明二者的内在联系如何促进癌细胞迁移。本研究结果显示：尽管糖酵解（glycolysis）可驱动受限微通道内的单细胞迁移，但快速迁移细胞与慢速迁移细胞对糖酵解及氧化磷酸化（oxidative phosphorylation）抑制剂的迁移敏感性存在显著差异。对高迁移率与低迁移率亚群（MDA+、MDA-）进行表型分选后发现，更偏向间充质表型的高迁移率MDA+亚群优先利用糖酵解供能，而更偏向上皮表型的低迁移率MDA-亚群则依赖线粒体呼吸（mitochondrial respiration）获取能量。上述表型具有可塑性：通过调控糖代谢或上皮间质转化（EMT），可使MDA+亚群的糖酵解活性、间充质特性与迁移能力降低；同时可使MDA-亚群的糖酵解活性、间充质特性与迁移能力提升。本研究结果揭示了EMT与糖代谢之间的内在关联，相关内容详见……。本研究数据由Reinhart-King实验室的设备与材料采集完成，实验操作严格遵循已发表论文中STAR Methods章节的规范流程。数据经Fiji/ImageJ软件处理后录入Excel表格，需使用微软Excel（Microsoft Excel）进行读取与编辑。