Somatic hypermutation in adult and aged mice

Somatic hypermutation sequences associate with the manuscript "Estradiol Mediates Greater Germinal Center Responses to Influenza Vaccination in Female than Male Mice" currently in press at mBio. Data include JH4-intron sequencing from aged male and female mice and adult male and female mice after vaccination and boosting with Flu antigen. Sequences were trimmed to remove primer sequence and contain a small fragment of the FW3 of the IgH V1 family in mice and end ~500 bp downstream of JH4. Sequences were isolated from germinal center B cells (CD19+CD38-GL7+), amplified by nested PCR, and sanger sequenced.

本数据集涉及体细胞超突变（Somatic hypermutation）序列，其关联的论文《雌二醇介导雌性小鼠比雄性小鼠对流感疫苗接种产生更强的生发中心应答（Estradiol Mediates Greater Germinal Center Responses to Influenza Vaccination in Female than Male Mice）》目前已被mBio期刊接收待刊。数据集包含来自老年雌雄小鼠以及经流感抗原免疫接种并加强免疫后的成年雌雄小鼠的JH4内含子（JH4-intron）测序数据。所获取的序列已完成引物序列修剪，包含小鼠免疫球蛋白重链V1（IgH V1）家族框架区3（FW3）的一段小片段，且序列末端位于JH4下游约500个碱基对（bp）处。该类序列从生发中心B细胞（CD19+CD38-GL7+）中分离得到，经嵌套式聚合酶链反应（nested PCR）扩增后进行桑格测序（Sanger）。