Lower airway microbiota in COPD and healthy controls

The lower airway microbiota in patients with chronic obstructive pulmonary disease (COPD) are likely altered compared with the microbiota in healthy individuals. Information on how the microbiota is affected by smoking, the use of inhaled corticosteroids (ICS), and COPD severity is still scarce. In the MicroCOPD Study, participant characteristics were obtained through standardised questionnaires and clinical measurements at a single centre from 2012 to 2015. Protected bronchoalveolar lavage samples from 97 patients with COPD and 97 controls were paired-end sequenced with the Illumina MiSeq System. Data were analysed in QIIME 2 and R. Alpha-diversity was lower in patients with COPD than controls (Pielou evenness: COPD=0.76, control=0.80, p=0.004; Shannon entropy: COPD=3.98, control=4.34, p=0.01). Beta-diversity differed with smoking only in the COPD cohort (weighted UniFrac: permutational analysis of variance R2=0.04, p=0.03). Nine genera were differentially abundant between COPD and con..., , , # Lower airway microbiota in COPD and healthy controls

#### This note was written by Solveig Tangedal, on Nov 29th, 2023, and edited on Dec 17th, 2024.

We have submitted deidentified participant data (metadata) in .xlsx and .txt format(**COPDvCMetadata.xlsx COPDvCMetadata.txt**),
Amplicon sequencing variants (ASV) tables in qza format for all ASVs and for ASVs eligible for differential abundance analyses (**COPDvC_ASV.qza COPDvCAncomASV.qza**)
Representative sequences tables in qza format for all ASVs (**COPDvCRepSeq.qza**),
Rooted phylogenetic trees in qza format (**COPDvC_rootedtree.qza**),
Taxonomy tables in qza format (**COPDvC_Taxonomy.qza**),
QIIME 2 code in txt format (**COPDvsControl_Q2_code.txt**),
R code in RScript format (**COPDvControl_Rcommand.R**),
and the manuscript abstract in docx format (**ABSTRACT.docx**)

The MicroCOPD study is a large study, with amplicon (16S rRNA) sequencing of more than 3000 samples over > 30 RUNs.

The airways samples number > 2500, and ...