iGABASnFR data from: Co-release of GABA and ACh from medial olivocochlear neurons as a fine regulatory mechanism of cochlear efferent inhibition

During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead exhibit spontaneous activity. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs, regulating their firing pattern which is crucial for the correct development of the auditory pathway. Although the MOC-IHC is a cholinergic synapse, previous evidence indicates the widespread presence of gamma-aminobutyric acid (GABA) signaling markers, including presynaptic GABAB receptors (GABABR). In this study, we explore the source of GABA by optogenetically activating either cholinergic or GABAergic fibers. The optogenetic stimulation of MOC terminals from GAD;ChR2-eYFP and ChAT;ChR2-eYFP mice evoked synaptic currents in IHCs that were blocked by α-bungarotoxin. This suggests that GABAergic fibers release ACh and activate α9α10 nicotinic acetylcholine receptors (nAChRs). Additionally, MOC cholinergic fibers release not o..., , # iGABASnFR data from: Co-release of GABA and ACh from medial olivocochlear neurons as a fine regulatory mechanism of cochlear efferent inhibition Dataset DOI: [10.5061/dryad.4tmpg4fq5](https://doi.org/10.5061/dryad.4tmpg4fq5) ## Description of the data and file structure We have submitted our raw iGABASnFR optical detection data to support the Castagnola et al 2025 manuscript. **Notes:** Data and File Structure: All raw data files contained in the .zip file “Castagnola_et_al_iGABASnFR.zip” Excel file “Castangola et al iGABASnFR file list final” contains a list of file names, types, and additional details for the dataset. Nikon files are all Nikon LIM .nd2 file type, but are noted in the attached excel file as “Nikon .nd2 LIM images” file type. .nd2 files can be viewed in Nikon Elements viewers or ImageJ. Sharing / Access information: Please contact [[e-mail]](mailto:[e-mail]) with any questions. Folder: “Castagnola et al iGABASnFR” contains Nikon...,在发育阶段，哺乳动物耳蜗内的内毛细胞(inner hair cells, IHCs)对声学刺激无响应，反而呈现自发放电活动。在此同期，源自内侧橄榄耳蜗复合体(medial olivocochlear complex, MOC)的神经元会暂时性向内毛细胞形成神经支配，调控其放电模式，这对听觉通路的正常发育至关重要。尽管MOC-IHC属于胆碱能突触，但已有研究证据显示该通路广泛存在γ-氨基丁酸(gamma-aminobutyric acid, GABA)信号标志物，包括突触前GABAB受体(GABABR)。本研究通过光遗传激活胆碱能或γ-氨基丁酸能纤维，探究了GABA的来源。对GAD;ChR2-eYFP和ChAT;ChR2-eYFP小鼠的MOC末梢进行光遗传刺激，可在内毛细胞中诱发出突触电流，且该电流可被α-银环蛇毒素阻断。这提示γ-氨基丁酸能纤维会释放乙酰胆碱(ACh)，并激活α9α10烟碱型乙酰胆碱受体(nicotinic acetylcholine receptors, nAChRs)。此外，MOC胆碱能纤维不仅会释放[原文截断]。 # 数据集来源：内侧橄榄耳蜗神经元共释放GABA与ACh作为耳蜗传出抑制的精细调控机制 数据集DOI：[10.5061/dryad.4tmpg4fq5](https://doi.org/10.5061/dryad.4tmpg4fq5) ## 数据与文件结构说明 我们已提交原始iGABASnFR光学检测数据，以支撑Castagnola等人2025年的研究论文。 **备注：** 数据与文件结构：所有原始数据文件均存储于压缩包"Castagnola_et_al_iGABASnFR.zip"中。 Excel文件"Castangola et al iGABASnFR file list final"包含本数据集的文件名、文件类型及其他详细信息列表。 尼康格式文件均为尼康LIM .nd2文件类型，在附件Excel文件中被标注为"Nikon .nd2 LIM images"文件类型。.nd2文件可通过尼康Elements Viewer或ImageJ软件查看。 共享与获取信息：如有任何疑问，请联系[[e-mail]](mailto:[e-mail])。 文件夹"Castagnola et al iGABASnFR"包含尼康[原文截断]数据