Data supporting development and validation of liquid chromatography tandem mass spectrometry method for the quantitative determination of bile acids in feces

The data presented here support the optimization of feces sample extraction for sensitive quantification of bile acids in human feces using LC-MS/MS. The dataset includes:
Comparison between recovery of bile acids from (a) dried feces, (b) dried feces spiked with deuterated internal standards prior to drying, and (c) wet feces
Short-term stability data for bile acids standard solutions and fecal extracts containing bile acids after 24 h storage at room temperature and at 6°C
Data for multiple freeze-thaw cycles on bile acids recovery in both standard solutions and fecal samples
Bile acids recovery from cold and warm extractions data

本数据集可为利用液相色谱-串联质谱（LC-MS/MS）对人类粪便中的胆汁酸进行高灵敏定量分析时，优化粪便样本提取流程提供数据支撑。本数据集包含以下内容：1. 三类样本的胆汁酸回收率对比：(a) 干燥粪便样本、(b) 干燥前添加氘代内标的干燥粪便样本、(c) 湿粪便样本；2. 胆汁酸标准溶液以及含胆汁酸的粪便提取物，分别于室温与6℃条件下储存24小时后的短期稳定性数据；3. 标准溶液与粪便样本经多次冻融循环后对胆汁酸回收率影响的相关实验数据；4. 低温提取与温热提取两种方式下，粪便样本的胆汁酸回收率数据。