Co-Conservation of Synaptic Gene Expression and Circuitry in Collicular Neurons

we compared the molecular and cellular organization of superior colliculus in mice, tree shrews, and humans. Our findings reveal that the SC has evolved by conserving neuron subtypes, synaptic genes, and circuitry, while diversifying ciliary gene expression and an inhibitory neuron subtype The sSC was micro-dissected approximately by knife cuts, processed into a single-nuclei suspension and prepared for sequencing with 10X Chromium Next GEM 3 reagent kit v3.1 according to the manufacturers protocol