The Glucocorticoid Receptor couples endocrine signalling to synaptic activity dependent gene transcription via a metabotropic glutamate receptor-regulated pathway in zebrafish - BisPCR2 data

The Glucocorticoid Receptor (GR) co-ordinates metabolic and behavioural responses to stressors. We hypothesised that GR influences behaviour by modulating epigenetic and transcriptional processes in the brain. Using the zebrafish as a model organism, the brain methylomes of wild-type and grs357 mutant adults were analysed and GR-sensitive, differentially methylated regions (GR-DMRs) were identified. DMRs linked to genes with biologically salient functions were selected for confirmatory deep sequencing analysis in wild-type and GR mutant 5dpf larval heads, using the BisPCR2 technique (Bernstein et al., 2015). Our results demonstrate that the genes fkbp5 and aplp1 exemplify two distinct modes of GR-regulated, glucocorticoid-responsive DNA methylation in the brain. Overall design: 96 larval head BisPCR2 samples (24 GRWT, 24 GRWT-BM, 24 GRmut, 24 GRmut-BM)