Functional profiling and visualization of the sphingolipid metabolic network in vivo

This dataset contains a comprehensive collection of z-stack confocal images from whole-mount adult Drosophila melanogaster brains. The images document the detailed cell-type expression patterns of 52 genes essential for sphingolipid metabolism. The expression of each gene was visualized using the GAL4-UAS binary system and immunostainings. Specific GAL4 reporter lines (C-terminus 3XHA-T2A-GAL4 insertion), which faithfully recapitulate the endogenous expression of the target genes, were used to drive a nuclear red fluorescent protein (UAS-mCherry-nls). Anti-Elav and anti-Repo stainings were used to label neurons and glia, respectively. Anti-HA stainings were used to visualize protein distributions.本数据集收录了一套完整的成年黑腹果蝇（Drosophila melanogaster）全脑整装标本的共聚焦Z-stack图像集合。该图像集记录了52个参与鞘脂代谢的核心基因的详细细胞类型表达谱。各基因的表达情况通过GAL4-UAS二元表达系统与免疫染色技术实现可视化。本研究采用了一类特异性GAL4报告品系：该品系通过在靶基因C端插入3XHA-T2A-GAL4序列，可忠实重现靶基因的内源表达模式，以此驱动核定位红色荧光蛋白（UAS-mCherry-nls）的表达。分别采用抗Elav抗体（Anti-Elav）染色与抗Repo抗体（Anti-Repo）染色标记神经元与神经胶质细胞。抗HA抗体（Anti-HA）染色则用于展示蛋白的分布情况。