Organelle calcium-derived voltage oscillations in pacemaker neurons drive the motor program for food-seeking behavior in Aplysia

An atypical neuronal pacemaker mechanism, based on rhythmic intracellular calcium store release in an identified pair of interneurons (B63) and resulting oscillation of the neurons’ membrane potential, acts as an autonomous releaser for the irregular occurrences of the motor program for food-seeking behavior in Aplysia. The rhythmic variations of the membrane potential in B63 neurons were analyzed by Fast Fourier Transform (FFT) analysis in a cycle period bandwidth of 512 s to 8 s. The resulting power spectral density periodograms were used to identify oscillation periods of peak magnitude. The periodograms were computed from the FFT frequency spectrograms by converting the frequency band (in Hz) to its reciprocal, period (in secs). These analysis were performed in isolated buccal ganglia preparations bathed in artifical sea water (ASW), in ‘Low Ca+Co’ saline to block chemical synapses, or in ‘Low Ca+Co’ saline and after an intracellular injection of organelle membrane calcium channel b...一种非典型神经元起搏机制，基于一对已鉴定的中间神经元（B63）内的节律性细胞内钙库释放，并伴随神经元膜电位的振荡，该机制可作为自主触发因子，介导海兔（Aplysia）觅食行为运动程序的不规则发生。本研究采用快速傅里叶变换（Fast Fourier Transform, FFT）对B63神经元的膜电位节律变化进行分析，分析的周期带宽范围为512秒至8秒。所得的功率谱密度周期图被用于识别峰值幅度对应的振荡周期。该周期图通过将频带（单位为赫兹）转换为其倒数即周期（单位为秒），从FFT频谱图中计算得到。相关分析在离体颊神经节标本中开展，实验条件分别为：置于人工海水（Artificial Sea Water, ASW）中、置于“低钙+钴”生理盐水中以阻断化学突触，或置于“低钙+钴”生理盐水中并经细胞内注射细胞器膜钙通道b……