Enhancing reproducibility in stable isotope analysis (SIA) of fish eye lenses: A comparison between lamina number and diameter

Analyzing stable isotopes in archival tissues, such as fish eye lenses, is used to document shifts in feeding ecology, diet, habitat use, and to reconstruct life history. Fish eye lenses grow sequentially throughout their ontogeny, resulting in a structure of multiple layers, or laminae. These laminae represent the chronology of the fish's life, much like tree rings. Lenses are protein-rich, which makes them an ideal structure for analyzing light isotopes such as δ¹³C, δ¹⁵N, and δ34S. These light isotopes are primarily integrated into the lens tissue through the fish's diet. As research begins to emerge using eye lenses to reconstruct the life histories of fishes, the need for a reproducible method of delamination grows. For this study, two different researchers peeled lenses from each eye of the same adult Chinook salmon (Oncorhynchus tshawytscha) (n=10 fish). Lens lamina number, diameter (mm), and mass (mg) of each lamina were recorded. Laminae were then submitted for isotopic analysi..., Metadata of eye lens stable isotope data (CN) of 10 2018 fall run Chinook salmon. Fish-specific metadata (e.g., fork_length, site_id) is given and collected by CDFW. Lens-specific metadata (e.g., layer_no, lens_dm) is collected during sample processing. Isotope data is generated by the Stable Isotope Facility at the University of California, Davis. , , # Enhancing reproducibility in stable isotope analysis (SIA) of fish eye lenses: A comparison between lamina number and diameter

[https://doi.org/10.5061/dryad.8cz8w9h1s](https://doi.org/10.5061/dryad.8cz8w9h1s)

## Description of the data and file structure

Metadata of eye lens stable isotope data (CN) of 10 2018 fall run Chinook salmon. Fish-specific metadata (e.g., fork length, site) is given and collected by CDFW. Lens-specific metadata (e.g., layer number, lens diameter) is collected during sample processing. Isotope data is generated by the Stable Isotope Facility at the University of California, Davis. 

### Files and variables

#### File: Lens\_Methods\_Metadata.csv

**Description:** Fish eye lens isotope (C,N) data used in Chu and Li, et al.  A comparison between lamina number and diameter

##### Variables

* lab_id: unique ID given to each individual fish
* researcher: Researcher who processed the lens sample
* layer_no: Lamina number assigned during dissection
* lens_dm: Di...,