Supplementary file 1_A mechanokinetic actomyosin model predicts different orthophosphate sensitivities of force and ATP turnover rate during isometric muscle contraction.docx

The release of the ATP hydrolysis product, orthophosphate (Pi), from the myosin active site, together with force-generating structural changes, is central to actomyosin energy transduction, but the temporal order of these events remains unclear. A range of data, interpreted using simple kinetic schemes (that do not account for varying cross-bridge strains) suggests that force generation is closely associated with the attachment of the myosin head to actin, preceding Pi-release. However, the addition of a branched pathway to the kinetic scheme is needed to account for the lower sensitivity of the isometric ATP-turnover rate to Pi compared with that of force. In contrast, a branched pathway does not appear necessary if the data are analyzed using a mechanokinetic model that incorporates the myosin strain distribution. Here, we corroborated this idea using a model in which Pi-release from the active site precedes the force-generating power-stroke. We explain the effect based on two components underlying the reduction in isometric force with increased [Pi]. The larger component arises from pre-power-stroke cross-bridges with high large elastic strain, whereas the smaller component results from cross-bridges attaching with low elastic strain. Because only the latter myosin heads undergo ATPase cycles, force exhibits greater Pi-sensitivity than ATPase activity. Changes in model parameter values that minimize the width of the cross-bridge strain distribution do not eliminate the difference in Pi-sensitivity between isometric force and ATPase. Such changes, including reduced actin affinity in a pre-power-stroke state, also lead to a proportional reduction in isometric force and in the number of attached cross-bridges with increased [Pi]. In conclusion, our data suggest that a mechanokinetic model explains the combined changes in isometric force, ATPase activity, and the number of attached cross-bridges with varied [Pi] more directly than apparently simpler kinetic schemes. A central feature of these results is the explicit demonstration of two components of isometric force with different physiological roles.