The ontogeny of eDNA shedding during early development in Chinook Salmon

We conducted a lab study to explore eDNA shedding during early life history (from fertilized eggs until near yolk sac absorption) in Chinook Salmon at three biomasses: 10 eggs, 100 eggs, and 1,000 eggs, and each egg biomass was replicated (indicated as replicates 1 and 2). Water samples were collected at several time points before, during, and after hatch for detection of Chinook Salmon DNA. On days when water samples were collected, we counted the number of dead eggs and the number of viable eggs hatched (identified as larvae emerged from eggs) until hatching was completed. The dataset represents the results from testing water samples for Chinook Salmon DNA using a quantitative polymerase chain reaction (qPCR) assay and the number of dead eggs and the number of viable eggs hatched.

本研究开展了一项室内实验室实验，旨在探究奇努克鲑鱼（Chinook Salmon）早期生活史阶段（从受精卵至卵黄囊即将吸收完成时期）的环境DNA（environmental DNA, eDNA）释放规律。实验设置3种卵生物量梯度：10枚卵、100枚卵及1000枚卵，每组卵生物量均设置2个生物学重复（记为重复组1与重复组2）。在孵化前、孵化期间及孵化后的多个时间点采集水样，用于检测奇努克鲑鱼DNA。在采集水样的当日，统计死卵数量与孵出活卵（即从卵中孵出的幼体）的数量，直至所有孵化过程完成。本数据集包含通过定量聚合酶链式反应（quantitative polymerase chain reaction, qPCR）检测水样中奇努克鲑鱼DNA的实验结果，以及死卵数量、孵出活卵数量的统计数据。