Transcription Factors Sp8 and Sp9 Coordinately Regulate Olfactory Bulb Interneuron Development [RNA-seq 2]

Purpose: To asses changes in gene expression profiles from the P30 wild type littermate control olfactory bulbs and conditional double knockout olfactory bulbs of hGFAP-Cre; Sp8 Flox/Flox; Sp9 Flox/Flox mice. Methods: Total RNA was isolated and sequenced from the olfactory bulbs of the P30 wild type littermate controls or hGFAP-Cre; Sp8 Flox/Flox; Sp9 Flox/Flox mice in tetrad using an illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered changed which performed fold-change>=2 and FDR<=0.05. Changed genes were then filtered to reveal the downstream targets of Sp8 and Sp9. Results: 31 genes were significantly increased and 74 genes were significantly decreased in expression level due to the loss of Sp9 and Sp8 expression. Total RNA was isolated and sequenced from the olfactory bulbs of the P30 wild type littermate controls or hGFAP-Cre; Sp8 Flox/Flox; Sp9 Flox/Flox mice in tetrad using an illumina high-seq 2500.研究目的：评估P30天龄野生型同窝对照小鼠嗅球与hGFAP-Cre; Sp8 Flox/Flox; Sp9 Flox/Flox条件性双敲除小鼠嗅球的基因表达谱变化。 方法：采用Illumina HiSeq 2500测序平台，对四组生物学重复的P30天龄野生型同窝对照小鼠及hGFAP-Cre; Sp8 Flox/Flox; Sp9 Flox/Flox小鼠的嗅球组织总RNA进行提取与测序。原始测序数据通过TopHat进行分析。当基因的差异倍数≥2且错误发现率（False Discovery Rate, FDR）≤0.05时，将其判定为差异表达基因。随后对差异表达基因进行筛选，以鉴定Sp8与Sp9的下游靶基因。 结果：因Sp8与Sp9表达缺失，嗅球中31个基因的表达水平显著上调，74个基因的表达水平显著下调。采用Illumina HiSeq 2500测序平台，对四组生物学重复的P30天龄野生型同窝对照小鼠及hGFAP-Cre; Sp8 Flox/Flox; Sp9 Flox/Flox小鼠的嗅球组织总RNA进行提取与测序。