Currently available methods do not sample the entire tRNA pool or lack specificity for tRNAs. Combining and optimizing high-throughput sequencing methods, we developed Lotte-seq a highly specific prot
tRNAs decoding standard 20 AA 61 Selenocysteine tRNAs (TCA) 0 Possible suppressor tRNAs (CTA,TTA,TCA) 0 tRNAs with undetermined or unknown isotypes 0 Predicted pseudogenes 1 Total tRNAs 62
tRNAs decoding standard 20 AA 43 Selenocysteine tRNAs (TCA) 0 Possible suppressor tRNAs (CTA,TTA,TCA) 0 tRNAs with undetermined or unknown isotypes 0 Predicted pseudogenes 0 Total tRNAs 43
Several pieces of evidence suggest that small RNA degradation products together with tRNase ZL appear to form another layer of the whole gene regulatory network. The degraded RNA such as a 5′-half-tRN
