Bcl11b prevents catastrophic autoimmunity by controlling multiple aspects of regulatory T-cell gene expression program [ATAC-seq]

In order to investigate whether Bcl11b mediates any chormatin accebility in Treg cell, we performed ATAC-Seq from Bcl11b sufficient (WT) and Bcl11b deficient Treg(KO) cells.We used 100,000 cells for each experiment. Genome wide occupancy profiling followed by high throughput sequencing for each conditions (3 replicates for Bcl11b WT Treg, 3 replicates for Bcl11b KO Treg) was done using Illumina HiSeq 2500.