Expression data from a malaria vaccine trial (HG-U133A2.0 and U133 Plus 2.0)

Volunteers were assessed at study entry, the day of the third vaccination and 24, 72 hours, two weeks after vaccination, and 5 days after challenge. 13/39 vaccinees were protected and 26/39 were not protected. Eleven vaccinees exhibited delayed onset of parasitemia. All infectivity controls developed parasitemia. Prediction Analysis of Microarrays (PAM-R) identified genes corresponding with protection. Gene Set Enrichment Analysis (GSEA) identified sets of genes associated with protection after the third immunization, before challenge. After the third immunization, prior to challenge, differential expression of genes in the immunoproteasome pathway distinguished protected and non protected persons Samples were collected at study entry, the day of third vaccination, 24 and 72 hours post the third vaccination and two weeks post the third vaccination as well as 5 days post challenge. Both longitudinal and cross-sectional analysis were carried out to investigate expression profiles associated with vaccine effects and vaccine efficacy. This dataset contains 215 samples from HG-U133 A 2.0 platform and 39 samples from HG Plus2.0 platform.

搜集汇总

背景与挑战

背景概述

该数据集来源于一项疟疾疫苗试验，包含234个样本（215个来自HG-U133 A2.0平台，39个来自HG Plus2.0平台），在多个时间点（入组、第三次疫苗接种前后及攻击后）采集。通过PAM-R和GSEA分析，识别出与保护相关的基因和免疫蛋白酶体途径的差异表达，旨在研究疫苗效果和效力的表达谱。