SNV analysis of the viral population (CSFV_Roesrath_P5) by NGS deep sequencing.

a Two independent populations of PCR products obtained from the viral population (CSFV_Roesrath_P5) were NGS deep sequenced using the FLX and Ion PGM platforms. SNV frequencies are shown, as percentages, for both datasets combined, called by both Lofreq and V-Phaser 2 (SNV Total), followed by the FLX and Ion PGM SNV percentages respectively. SNVs frequencies called only with V-phaser 2 are shown in parenthesis.b This column indicates which cDNA clone haplotype the SNV belongs to. Colour codes correspond to haplotypes used in the phylogeny and the mutation frequency distribution in Fig 2A and 2B. P: Purple; B: Blue; L: Light blue; Y: Yellow; O: Orange; R: Red; G: Green.SNV analysis of the viral population (CSFV_Roesrath_P5) by NGS deep sequencing.