Diagnosis of childhood bacterial and viral infection using host RNA expression [Discovery set]. Homo sapiens

Genome-wide analysis of transcriptional profiles in children <17 years of age with bacterial or viral infections or with clinical features suggestive of infection. Overall design: Patients were recruited at or after presentation to hospital. Main inclusion criteria were presence of illness of sufficient severity to warrant blood tests for clinical reasons, in child aged <17 years. Blood was collected into PAX gene tubes (PreAnalytiX). Total RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). Labeled cRNA was hybridized to Illumina Human HT-12 v4 Beadchips (Discovery set) or Illumina Human HT-12 V3 Beadchips (Validation set). Numbers of arrays analysed were as follows: Definite Bacterial infection (n=52), Definite Viral infection (n=92), Probable Bacterial infection (n=42), Probable Viral infection (n=5), infections of uncertain bacterial or viral aetiology (n=49), Healthy controls (n=52).