Per- and Polyfluoroalkyl Substances Cause Changes in Gene Expression within HepG2 Cells Individually and as Equimolar Mixtures in Concentration-Response

This study set out to evaluate the hypothesis that PFAS exposure alters EV regulation via upstream molecular mediators of EV-based alterations through cellular transcriptomics assessment in a concentration-response manner. To evaluate this hypothesis, HepG2 liver cells were treated in concentration-response with individual PFOS, PFOA, or PFHxA, in addition to an equimolar PFAS mixture. Global gene expression was measured. These analyses set out to evaluate concentration-dependent changes in mRNA signatures within HepG2 immortalized liver cells exposed to individual PFOS, PFOA, PFHxA or an equimolar mixture containing all three PFAS. Gene expression data from HepG2 immortalized liver cell line following 24 hr PFAS exposures We tested an equimolar mixture containing three PFAS compounds: PFHxA, PFOA, and PFOS; with each individual chemical at a concentration of 2.5uM, 10uM, 25uM, or 35uM. We additionally tested PFOS, PFOA, and PFHxA individually at concentrations of 2.5uM, 10uM, 25uM, or 35uM. These PFAS conditions were used to treat HepG2 cells with a 24 hour time-point. RNA was subsequently isolated from HepG2 cell lysates. mRNA expression was measured using an array to evaluate changes in expression associated with exposure.