Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe from 27/02/2003 to 04/03/2003 (Dumont d'Urville - Hobart)

These data were collected on the RV L'Astrolabe (platform code: FHZI) from 27/02/2003 to 04/03/2003 on a trip from Dumont d'Urville to Hobart.

Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).

Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).

本数据集采集于星盘号研究船（RV L'Astrolabe，平台编码：FHZI），采集时段为2003年2月27日至2003年3月4日，对应航次为从迪蒙·迪维尔（Dumont d'Urville）前往霍巴特（Hobart）的航行。

光系统II（photosystem II，PSII）的最大光化学效率，亦被称为PSII的最大量子产额（Fv/Fm），现已成为浮游植物研究中应用最为广泛的荧光参数之一。该参数表征潜在光化学效率，即光合装置捕获的光能被用于光化学反应的概率。研究表明，Fv/Fm可对水体物理与化学性质的变化产生即时响应，常被用作诊断浮游植物整体健康状态与生理活性的指标。结合PSII吸收截面积与叶绿素浓度数据，该参数可用于水体初级生产力的测定（Cheah等，2011，《深海研究（Deep Sea Research）》）。

采样海水取自3米深度，通过船舶专用清洁海水管路连续供给。快速重复率（Fast Repetition Rate，FRR）荧光产量采用暗适应状态下的连续测量模式，测量间隔为1分钟，测量前需经15分钟暗适应。所用闪光序列包含两组闪光：100次亚饱和微闪光（闪光时长1.1 μs，闪光间隔2.8 μs）与20次弛豫微闪光（闪光时长1.1 μs，闪光间隔51.6 μs）。